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Proceedings Paper

In-situ localization of a photosensitizer as evaluated by vital microscopy
Author(s): Pal Kristian Selbo; Tor Brustad; Torhild Voss; Johan Moan; Kristian Berg
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Paper Abstract

The present study is based on the use of vital microscopy and a charge couple device camera for localization of a porphyrin in normal and neoplastic tissue in nude athymic Balb/c nu/nu mice. Granular tissue was allowed to grow into the space between two optical widows, separated by a 40 micrometer thick spacer in a transparent chamber. WiDr cells derived from a primary adenocarcinoma of the rectosigmoid colon, were used as a tumor model. Tumor cells were grown in vitro as spheroids and transplanted to the tissue in the chamber. The photosensitizer, meso(tetra-phenyl)porphine with two sulfonate groups on adjacent phenylrings (TPPS2a), was injected i.p. to a concentration of 25 mg kg-1, and the porphyrin localization was evaluated in vivo and in situ by a fluorescence microscopical technique. A significant difference between normal and neoplastic tissue was observed, with a much faster uptake of the photosensitizer in tumor tissue than in normal tissue.

Paper Details

Date Published: 29 December 1997
PDF: 8 pages
Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); doi: 10.1117/12.297802
Show Author Affiliations
Pal Kristian Selbo, Norwegian Radium Hospital (Norway)
Tor Brustad, Norwegian Radium Hospital (Norway)
Torhild Voss, Norwegian Radium Hospital (Norway)
Johan Moan, Norwegian Radium Hospital (Norway)
Kristian Berg, Norwegian Radium Hospital (Norway)

Published in SPIE Proceedings Vol. 3191:
Photochemotherapy: Photodynamic Therapy and Other Modalities III
Kristian Berg; Benjamin Ehrenberg; Zvi Malik; Johan Moan; Abraham Katzir, Editor(s)

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