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Proceedings Paper

Collagen-gel-induced resistance of overlying keratinocyte cultures to photosensitization
Author(s): John C. Katsantonis; Savas K. Georgiou; Mary G. Providaki; John G. Vlahonikolis; Andronicki D. Tosca
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Paper Abstract

In the present study it was found that human keratinocytes grown on collagen substrate, exhibited increased resistance to the hematoprophyrin-mediated photodynamic treatment, in comparison to keratinocytes grown on Petri dishes without collagen. Interestingly, no protection was afforded by the collagen gel to the cells in the corresponding control ('hematoprophyrin only' and 'light only') experiments. This observation was found to be independent of light dose and drug concentration, and the relative degree of resistance was the same for both normal and malignant cells. The degree of keratinocyte resistance was found to be closely related to the duration of cell attachment on the collagen substrate. These results are indicative of an active interference of collagen gel with the cellular evolution of the photodynamic phenomenon and they are also suggestive of variation in the photodynamic treatment efficacy according to the cellular environment.

Paper Details

Date Published: 29 December 1997
PDF: 11 pages
Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); doi: 10.1117/12.297792
Show Author Affiliations
John C. Katsantonis, Heraklion Univ. General Hospital (Greece)
Savas K. Georgiou, FORTH/Institute of Electronic Structure and Laser (Greece)
Mary G. Providaki, Heraklion Univ. General Hospital (Greece)
John G. Vlahonikolis, Univ. of Crete Medical School (Greece)
Andronicki D. Tosca, Heraklion Univ. General Hospital (Greece)

Published in SPIE Proceedings Vol. 3191:
Photochemotherapy: Photodynamic Therapy and Other Modalities III
Kristian Berg; Benjamin Ehrenberg; Zvi Malik; Johan Moan; Abraham Katzir, Editor(s)

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