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Proceedings Paper

Picosecond infrared studies of protein vibrational modes: the amide I mode of myoglobin
Author(s): Kristen A. Peterson; James R. Engholm; Chris W. Rella; H. Alan Schwettman
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Paper Abstract

The population lifetime of the amide I vibration (v10 fundamental, ca. 1650 cm-1) in the protein myoglobin in D2O has been determined by picosecond infrared pump- probe spectroscopy using the Stanford mid-infrared free electron laser to be 1.3 +/- 0.2 ps. In a glass forming mixture of deuterated glycerol and D2O, the vibrational lifetime was found to increase from 1.3 +/- 0.2 ps at 310 K to 1.8 +/- 0.2 ps at 10 K. In addition to determining the time-scale of vibrational relaxation, we also observed multi-level vibrational excitation which has implications regarding the anharmonicity and homogeneous linewidth of the mode.

Paper Details

Date Published: 16 October 1997
PDF: 10 pages
Proc. SPIE 3153, Accelerator-Based Infrared Sources and Applications, (16 October 1997); doi: 10.1117/12.290259
Show Author Affiliations
Kristen A. Peterson, New Mexico State Univ. (United States)
James R. Engholm, Stanford Univ. (United States)
Chris W. Rella, Stanford Univ. (United States)
H. Alan Schwettman, Stanford Univ. (United States)


Published in SPIE Proceedings Vol. 3153:
Accelerator-Based Infrared Sources and Applications
Gwyn P. Williams; Paul Dumas, Editor(s)

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