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Proceedings Paper

Color image analysis of contaminants and bacteria transport in porous media
Author(s): Mehdi Rashidi; Jamshid Dehmeshki; Mohammad Farhang Daemi; Larry Cole; Eric Dickenson
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Paper Abstract

Transport of contaminants and bacteria in aqueous heterogeneous saturated porous systems have been studied experimentally using a novel fluorescent microscopic imaging technique. The approach involves color visualization and quantification of bacterium and contaminant distributions within a transparent porous column. By introducing stained bacteria and an organic dye as a contaminant into the column and illuminating the porous regions with a planar sheet of laser beam, contaminant and bacterial transport processes through the porous medium can be observed and measured microscopically. A computer controlled color CCD camera is used to record the fluorescent images as a function of time. These images are recorded by a frame accurate high resolution VCR and are then analyzed using a color image analysis code written in our laboratories. The color images are digitized this way and simultaneous concentration and velocity distributions of both contaminant and bacterium are evaluated as a function of time and pore characteristics. The approach provides a unique dynamic probe to observe these transport processes microscopically. These results are extremely valuable in in-situ bioremediation problems since microscopic particle-contaminant- bacterium interactions are the key to understanding and optimization of these processes.

Paper Details

Date Published: 24 October 1997
PDF: 11 pages
Proc. SPIE 3159, Algorithms, Devices, and Systems for Optical Information Processing, (24 October 1997); doi: 10.1117/12.284200
Show Author Affiliations
Mehdi Rashidi, Lawrence Livermore National Lab. (United States)
Jamshid Dehmeshki, Univ. of Nottingham (United Kingdom)
Mohammad Farhang Daemi, Univ. of Nottingham (United Kingdom)
Larry Cole, Texas A&M Univ. (United States)
Eric Dickenson, Lawrence Livermore National Lab. (United States)

Published in SPIE Proceedings Vol. 3159:
Algorithms, Devices, and Systems for Optical Information Processing
Bahram Javidi; Demetri Psaltis, Editor(s)

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