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Proceedings Paper

Native cellular fluorescence characteristics of normal and malignant epithelial cells from human larynx
Author(s): Diagaradjane Parmeswearan; Singaravelu Ganesan; R. Nalini; Prakasa Rao Aruna; V. Veeraganesh; Robert R. Alfano
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Paper Abstract

Many applications of native fluorescence spectroscopy of intrinsic biomolecules such as Try, Tyr, Phe, NADH and FAD are reported on both the characterization and the discrimination of malignant tissues from the normal. In the field of diagnostic oncology, extensive studies have been made to distinguish the normal from malignant condition in breast, cervix, colon and bronchus. From the studies made by Alfano and co-workers, it was found that the emission at 340 and 440 nm under UV excitation have shown statistically significant difference between normal and malignant tissues. As tissues are highly complex in nature, it is worth to known whether the changes arise from cells or from other extracellular tissue components, so as to enable us to have better understanding on the transformation mechanism of normal into malignant and to go for an improved approach in the effective optical diagnosis. In this context, the present study addresses the question of whether there are differences in the native cellular fluorescence characteristics between normal and malignant epithelial cells from human larynx. With this aim, the UV fluorescence emission spectra in the wavelength region of excitation between 270 - 310 nm and the excitation spectra for 340 nm emission were measured and analyzed. In order to quantify the altered fluorescence signal between the normal and malignant cells, different ratio parameters were introduced.

Paper Details

Date Published: 18 August 1997
PDF: 6 pages
Proc. SPIE 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II, (18 August 1997); doi: 10.1117/12.280222
Show Author Affiliations
Diagaradjane Parmeswearan, Anna Univ. (India)
Singaravelu Ganesan, Anna Univ. (India)
R. Nalini, King Institute of Preventative Medicine (India)
Prakasa Rao Aruna, Anna Univ. (India)
V. Veeraganesh, Anna Univ. (India)
Robert R. Alfano, CUNY/City College (United States)


Published in SPIE Proceedings Vol. 2979:
Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II
Britton Chance; Robert R. Alfano, Editor(s)

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