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Proceedings Paper

Use of a gain-modulating framing camera for time-resolved imaging of cellular phenomena
Author(s): Hiroyasu Itoh; Ariella Evenzahav; Katsuyuki Kinoshita; Yoshinori Inagaki; Hiroshi Mizushima; Akira Takahashi; Tsuyoshi Hayakawa; Kazuhiko Kinosita
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Paper Abstract

A gain modulating framing camera and its application towards the study of real time cellular phenomena is described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image- forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating frame camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We describe examples of application of the system towards the observation of various cellular phenomena.

Paper Details

Date Published: 18 August 1997
PDF: 8 pages
Proc. SPIE 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II, (18 August 1997); doi: 10.1117/12.280219
Show Author Affiliations
Hiroyasu Itoh, Hamamatsu Photonics KK (Japan)
Ariella Evenzahav, Columbia Univ. (United States)
Katsuyuki Kinoshita, Hamamatsu Photonics KK (Japan)
Yoshinori Inagaki, Hamamatsu Photonics KK (Japan)
Hiroshi Mizushima, Hamamatsu Photonics KK (Japan)
Akira Takahashi, Hamamatsu Photonics KK (Japan)
Tsuyoshi Hayakawa, Hamamatsu Photonics KK (Japan)
Kazuhiko Kinosita, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 2979:
Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II
Britton Chance; Robert R. Alfano, Editor(s)

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