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Proceedings Paper

Novel Archaea and Bacteria in a hydrologically isolated deep subsurface environment
Author(s): Darrell P. Chandler; Fred J. Brockman; James K. Fredrickson
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Paper Abstract

Total community DNA and RNA were extracted from a deep (188 m), saturated, mesophilic (17 degree(s)C), low biomass (104 cells g-1) subsurface paleosol. 16S rDNA was amplified by PCR using universal eubacterial or archaeal primers and cloned. Cloned 16S rDNA fragments were sequenced and subjected to phylogenetic analysis. A novel clade of Crenarchaea were discovered which are most closely related to a single clone recovered from an Fe-S hot spring (80 degree(s)C). Novel Bacterial sequences were also found which branch deeply in the universal phylogenetic tree in association with Chloroflexus, Deinococcus, and Thermus. Total RNA analysis suggested that the novel Crenarchaea were active in this environment. Geological and hydrologic properties of the sediment indicate that microbial and nutrient transport between individual strata are restricted, and are consistent with chemolithoautotrophic metabolisms of cultured Crenarchaea involving the H2/SO4 electron donor/acceptor couple. The physical properties of the surrounding lithologies suggest that these novel microorganisms are descendants of the microbial communities originally deposited with the sediment ca. 6 - 8 Mya. Identification of these novel, active microorganisms in an oligotrophic, hydrologically isolated subsurface system provides an opportunity and species-specific nucleic acid sequences and probes necessary to study long-term microbial survival in terrestrial subsurface environments.

Paper Details

Date Published: 11 July 1997
PDF: 10 pages
Proc. SPIE 3111, Instruments, Methods, and Missions for the Investigation of Extraterrestrial Microorganisms, (11 July 1997); doi: 10.1117/12.278808
Show Author Affiliations
Darrell P. Chandler, Pacific Northwest National Lab. (United States)
Fred J. Brockman, Pacific Northwest National Lab. (United States)
James K. Fredrickson, Pacific Northwest National Lab. (United States)

Published in SPIE Proceedings Vol. 3111:
Instruments, Methods, and Missions for the Investigation of Extraterrestrial Microorganisms
Richard B. Hoover, Editor(s)

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