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Proceedings Paper

Aerosol fluorescence spectrum analyzer for individual airborne biological particles
Author(s): Gang Chen; Ronald G. Pinnick; Steven C. Hill; Richard K. Chang; Paul Nachman; Gorden W. Videen
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Paper Abstract

We report the operation of an aerosol analyzer capable of measuring the fluorescence spectra of single micrometer-size bioaerosol particles. Aerosol particles in an air stream initially transverse a cw 488-nm 'trigger' laser beam where their elastic scattering and total fluorescence is measured with photomultipliers. When the elastic scattering and fluorescence signals meet certain criteria, a UV (266 nm) 'probe' laser is triggered and it illuminates the selected particles. The UV laser-excited spectra of particles are measured with the instrument's image-intensified CCD detector, gaged with signals from the trigger laser. We demonstrate the ability of the instrument to capture the fluorescence of single airborne biological particles. The results suggest that it may be possible to differentiate among biological particles based on their single particle fluorescence spectra.

Paper Details

Date Published: 30 May 1997
PDF: 9 pages
Proc. SPIE 3105, Chemical, Biochemical and Environmental Fiber Sensors IX, (30 May 1997); doi: 10.1117/12.276175
Show Author Affiliations
Gang Chen, Yale Univ. (United States)
Ronald G. Pinnick, Army Research Lab. (United States)
Steven C. Hill, Army Research Lab. (United States)
Richard K. Chang, Yale Univ. (United States)
Paul Nachman, Army Research Lab. (United States)
Gorden W. Videen, Army Research Lab. (United States)

Published in SPIE Proceedings Vol. 3105:
Chemical, Biochemical and Environmental Fiber Sensors IX
Robert A. Lieberman, Editor(s)

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