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Proceedings Paper

Development of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments
Author(s): Karen Y. Kavanagh; James Edward Walsh; J. Murphy; M. Harmey; M. A. Farrell; O. Hardimann; R. Perryman
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Paper Abstract

The continuing demand for non-invasive tools for use in clinical diagnosis has created the need for flexible and innovative optical systems which satisfy current requirements. We report the development of a microspectrophotometer system for use on mitochondrial respiratory pigments. This novel optical fiber set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Preliminary data is presented for the reduction of cytochrome-c by two methods. In the first, cytochrome-c was reduced in isolation using sodium dithionite. The second was an in-vivo simulation of the reduction of cytochrome-c using the mitochondrial extract from rat liver. The key features of the system are; front end adaptability, high sensitivity and fast scanning capabilities which are essential for the rapid biological reactions which are observed.

Paper Details

Date Published: 30 May 1997
PDF: 8 pages
Proc. SPIE 3105, Chemical, Biochemical and Environmental Fiber Sensors IX, (30 May 1997); doi: 10.1117/12.276138
Show Author Affiliations
Karen Y. Kavanagh, Dublin Institute of Technology (Ireland)
James Edward Walsh, Dublin Institute of Technology (Ireland)
J. Murphy, Univ. College Dublin (Ireland)
M. Harmey, Univ. College Dublin (Ireland)
M. A. Farrell, Richmond Institute for Neurology and Neurosurgery/Beaumont Hospital (Ireland)
O. Hardimann, Richmond Institute for Neurology and Neurosurgery/Beaumont Hospital (Ireland)
R. Perryman, Richmond Institute for Neurology and Neurosurgery/Beaumont Hospital (Ireland)


Published in SPIE Proceedings Vol. 3105:
Chemical, Biochemical and Environmental Fiber Sensors IX

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