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Proceedings Paper

Alstonine as a potential fluorescent marker for tiny tumor detection and imaging
Author(s): Pierre M. Viallet; Tuan Vo-Dinh; Jean-Marie Salmon; Wendi Watts; Emmanuelle Rocchi; Narayana R. Isola; Xavier Rebillard
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Paper Abstract

3,4,5,6,16,17-Hexadehydro-16-(methoxycarbolyl)-19(alpha) - methyl-20(alpha) -oxyohimbanium (alstonine) is a fluorescent alcaloid which is known to stain tumor cells more efficiently than normal. The interactions between alstonine and biological macromolecules were first investigated to provide the rationale for preferential labelling. Molecular filtration and spectrosfluorometric techniques with different macromolecules and isopolynucleotides have demonstrated that binding occurs only in the presence of uridyl rings. For the binding affect only the fluorescence intensity of alstonine it can be assumed that it involves only the side chain of the fluorescent compound. The capability for preferential staining was verified in culture using SK-OV-3 cells and rat hepatocarcinoma cells as tumor cells and Mouse fibroblasts or rat liver cells as controls. Techniques of image analysis have demonstrated the efficiency of cellular labelling even in aggregates of rat hepatocarcinoma. These experiments lead the way to the detection of tiny tumors developed on thin visceral walls, using a fiber optic device.

Paper Details

Date Published: 16 June 1997
PDF: 9 pages
Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); doi: 10.1117/12.275541
Show Author Affiliations
Pierre M. Viallet, Univ. de Perpignan (France)
Tuan Vo-Dinh, Oak Ridge National Lab. (United States)
Jean-Marie Salmon, Univ. de Perpignan (France)
Wendi Watts, Oak Ridge National Lab. (United States)
Emmanuelle Rocchi, Univ. de Perpignan (France)
Narayana R. Isola, Oak Ridge National Lab. (United States)
Xavier Rebillard, Clinique Beausoleil (France)

Published in SPIE Proceedings Vol. 2976:
Biomedical Sensing, Imaging, and Tracking Technologies II
Tuan Vo-Dinh; Robert A. Lieberman; Gerald G. Vurek; Abraham Katzir, Editor(s)

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