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Proceedings Paper

Raman measurement of glucose in bioreactor materials
Author(s): Yijing Xu; Joseph F. Ford; Charles K. Mann; Thomas J. Vickers; John M. Brackett; Kristen L. Cousineau; W. Gerard Robey
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Paper Abstract

The feasibility of using Raman spectroscopy to monitor the concentration of chemical species in a bioreactor has been examined. Successful operation of a bioreactor requires that nutrients and metabolic waste products be maintained within narrow ranges, and it is, therefore, important to provide accurate, reliable and timely measurement of the composition in the reactor. Raman spectroscopy offers the possibility of real time simultaneous monitoring of molecular components present in the millimolar and higher concentration range. Work reported here has focused on four analytes: glucose, glutamine, lactic acid and ammonia. Measurements have been made with a spectrograph providing a spectral window for simultaneous measurement of about 1800 cm-1 on a multichannel CCD detector. Most measurements were made with an argon ion laser emitting at 514.5 nm. Some measurements are reported with a solid state diode laser operating at 785 nm. Locally constructed inexpensive silica fiber-optic probes delivered the laser light and collected the scattered radiation. Spectra of the four analytes n buffer and reactor media have been obtained. Analytical curves have been constructed and limits of detection measured. Limits of detection in buffer media are about 1 mM. Results are reported for off-line measurements on material drawn from a bioreactor.

Paper Details

Date Published: 16 June 1997
PDF: 10 pages
Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); doi: 10.1117/12.275527
Show Author Affiliations
Yijing Xu, Florida State Univ. (United States)
Joseph F. Ford, Florida State Univ. (United States)
Charles K. Mann, Florida State Univ. (United States)
Thomas J. Vickers, Florida State Univ. (United States)
John M. Brackett, Abbott Labs. (United States)
Kristen L. Cousineau, Abbott Labs. (United States)
W. Gerard Robey, Abbott Labs. (United States)

Published in SPIE Proceedings Vol. 2976:
Biomedical Sensing, Imaging, and Tracking Technologies II
Tuan Vo-Dinh; Robert A. Lieberman; Gerald G. Vurek; Abraham Katzir, Editor(s)

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