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Proceedings Paper

Detection of fluorophore-labeled antibodies by surface-enhanced fluorescence on metal nanoislands
Author(s): Thomas G. M. Schalkhammer; Franz Rembert Aussenegg; Alfred Leitner; Harald Brunner; Gerhard Hawa; Christina Lobmaier; Fritz Pittner
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Paper Abstract

In fluorescence labelled immunosensing the discrimination of labels remaining in the bulk solution from labels bound to the analyte at the sensor surface is a basic optical problem. It is shown that application of surface enhanced fluorescence at a layer of noble metal nano-particles can increase the surface-to-background signal ratio. We explain the enhancement mechanism by an electrodynamic model and discuss the interaction between metal particle and fluorophore for the excitation and emission process. We show the principal guidelines for optimization of that processes. We find that the obtained discrimination power increases with decreasing intrinsic quantum efficiency of the fluorophore, suggesting the application of new classes of labels, namely low-quantum efficiency fluorophores. This theoretical finding is shown by a practical model experiment.

Paper Details

Date Published: 16 June 1997
PDF: 8 pages
Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); doi: 10.1117/12.275521
Show Author Affiliations
Thomas G. M. Schalkhammer, Univ. Wien (Netherlands)
Franz Rembert Aussenegg, Karl-Franzens-Univ. Graz (Austria)
Alfred Leitner, Karl-Franzens-Univ. Graz (Austria)
Harald Brunner, Karl-Franzens-Univ. Graz (Austria)
Gerhard Hawa, Univ. Wien (Austria)
Christina Lobmaier, Univ. Wien (Austria)
Fritz Pittner, Univ. Wien (Austria)

Published in SPIE Proceedings Vol. 2976:
Biomedical Sensing, Imaging, and Tracking Technologies II
Tuan Vo-Dinh; Robert A. Lieberman; Gerald G. Vurek; Abraham Katzir, Editor(s)

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