Share Email Print
cover

Proceedings Paper

Microcavitation and cell injury in RPE cells following short-pulsed laser irradiation
Author(s): Michael W. Kelly; Charles P. Lin
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Our research investigates the mechanism of retinal injury from short laser pulses. We used an ex-vivo porcine model and time resolved strobe imaging to examine mechanical effects associated with sub-nanosecond absorption by the retinal pigment epithelium (RPE). We present a unique method of imaging high speed event sin the RPE with a resolution of approximately 1 micrometers . Microcavitation bubbles were first imaged around single melanosomes, after irradiation with a 40 ps, 532 nm pulse. The threshold for bubble formation was 50 mJ/cm2. A few times threshold, stress waves could be observed around the melanosomes. At 0.68 J/cm2 we measured shock waves with an average velocity of 2700 m/s, 1 ns after irradiation. After exposures above 50 mJ/cm2, RPE cells were found to be non-viable. We compare these results with MVL data collected using live animal models.

Paper Details

Date Published: 16 June 1997
PDF: 6 pages
Proc. SPIE 2975, Laser-Tissue Interaction VIII, (16 June 1997); doi: 10.1117/12.275473
Show Author Affiliations
Michael W. Kelly, Wellman Labs. of Photomedicine, Massachusetts General Hospital, and Harvard Medical School (United States)
Charles P. Lin, Wellman Labs. of Photomedicine, Massachusetts General Hospital, and Harvard Medical School (United States)


Published in SPIE Proceedings Vol. 2975:
Laser-Tissue Interaction VIII
Steven L. Jacques, Editor(s)

© SPIE. Terms of Use
Back to Top