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Proceedings Paper

Far-ultraviolet absorption spectra of porcine and human corneas
Author(s): Annamarie Lembares; Xin-Hua Hu; Gerhard W. Kalmus
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Paper Abstract

We have measured the absorption spectra of porcine and human corneas in the far ultraviolet region from 350 nm to 190 nm. The samples were sectioned from frozen corneas into thin slices of 15 micrometers to 40 micrometers in thickness. The absorption spectra shows three distinct regions from 260 nm to 190 nm: a relatively weak absorption region from 260 nm to 240 nm; a region of steep increase from 240 nm to 220 nm; and a strong and slowly increasing absorption region from 220 nm to 190 nm. The linear absorption coefficients were determined to be 2130 +/- 310 (cm-1) at 220 nm, 2240 +/- 320 (cm-1) at 215 nm, 2300 +/- 330 (cm-1) at 210 nm, and 2410 +/- 370 (cm-1) at 193 nm for porcine corneas and 2300 +/- 480 (cm-1) at 220 nm, 2300 +/- 460 (cm-1) at 215 nm, 2320 +/- 470 (cm-1) at 210 nm, and 2340 +/- 450 (cm-1) at 193 nm for human corneas. A `window of ablation' in the far ultraviolet region between 220 and 190 nm was determined. Statistical analysis was conducted to correlate the far ultraviolet absorption between the porcine and human corneas. The effect of freezing on the far ultraviolet absorption also has been studied.

Paper Details

Date Published: 26 May 1997
PDF: 9 pages
Proc. SPIE 2971, Ophthalmic Technologies VII, (26 May 1997); doi: 10.1117/12.275123
Show Author Affiliations
Annamarie Lembares, East Carolina Univ. (United States)
Xin-Hua Hu, East Carolina Univ. (United States)
Gerhard W. Kalmus, East Carolina Univ. (United States)

Published in SPIE Proceedings Vol. 2971:
Ophthalmic Technologies VII
Pascal O. Rol; Karen Margaret Joos M.D.; Fabrice Manns, Editor(s)

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