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Proceedings Paper

Microelectrophoresis devices with integrated fluorescence detectors and reactors for high-throughput DNA sequencing
Author(s): Steven A. Soper; Sean M. Ford; Jack Davies; Daryl C. Williams; Benxu Cheng; J. Michael Klopf; Gina M. Calderon; Volker Saile
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Paper Abstract

This work describes the development of micro-devices for high-throughput DNA sequencing applications. Basically, two research efforts will be discussed; (1) fabrication and characterization of micro-reactors to prepare Sanger chain terminated DNA sequencing fragments on a nanoliter scale and; (2) x-ray photolithography of PMMA substrates for the high aspect ratio preparation of electrophoresis devices. The micro-reactor consisted of a 5'-biotinylated catfish olfactory gene, which was amplified by PCR, and attached to the interior wall of an aminoalkylisilane derivatized fused- silica capillary tube via a streptavidin/biotin linkage. Coverage of the interior capillary wall with biotinylated DNA averaged 77 percent. Stability of the anchored template under pressure and electroosmotic rinsing was favorable, requiring approximately 150 h of continuous rinsing to reduce the coverage by only 50 percent. The capillary micro- reactor was placed inside an air thermocycler to control temperature during Sanger ddNTP chain extension and directly coupled to a capillary separation column filled with a LPA solution via low dead volume capillary interlocks. The complimentary DNA fragments generated in the reactor were heat denatured from the immobilized template and directly injected onto a gel-filled capillary using electropumping for size fractionation and detection using NIR-LIF analysis. The total amount of termination fragments in the 31 nL reactor volume was estimated to be 5.2 X 1013 moles and sequencing was shown to produce read lengths on the order to 400 bases. Work will also be described concerning the development of micro-electrophoresis devices in x-ray sensitive photoresists using LIGA techniques. An electrophoresis device with an integrated fluorescence detector was constructed for the high resolution separation of DNA oligonucleotides. The choice of substrate for the electrophoresis was PMMA, due to its intrinsic low electroosmotic flow. Using x-ray lithography in PMMA substrates, the aspect ratios associated with the micromachining was estimated to be > 10,000:1.

Paper Details

Date Published: 22 May 1997
PDF: 11 pages
Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); doi: 10.1117/12.274366
Show Author Affiliations
Steven A. Soper, Louisiana State Univ. (United States)
Sean M. Ford, Louisiana State Univ. (United States)
Jack Davies, Louisiana State Univ. (United States)
Daryl C. Williams, Louisiana State Univ. (United States)
Benxu Cheng, Louisiana State Univ. (United States)
J. Michael Klopf, Louisiana State Univ. (United States)
Gina M. Calderon, Louisiana State Univ. (United States)
Volker Saile, Louisiana State Univ. (United States)

Published in SPIE Proceedings Vol. 2985:
Ultrasensitive Biochemical Diagnostics II
Gerald E. Cohn; Gerald E. Cohn; Steven A. Soper, Editor(s)

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