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Proceedings Paper

Compact upconverting phosphor detection system for wick assays
Author(s): Haris Riris; Jan E. Van der Laan; David E. Cooper; Karen M. Nashold; Clinton B. Carlisle; Luke V. Schneider
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Paper Abstract

We describe an integrated detection system based on upconverting phosphor particles bound to capture sites on the inside surfaces of rectangular wick capillaries. This deice can be used with either antibody or nucleic acid to detect specific micro-organisms. The system uses a high- power, 980 nm, semiconductor diode laser to illuminate 200 X 300 X 20 micrometers capture surfaces. The rectangular capillary wicks are held in a tray that is inserted into the detection system, positioning the capture surface at the object plane of the optical system. Phosphorescent light emitted from the capture surface is collected by a high numerical aperture microscope objective and directed through a series of filters onto either a CCD camera or a photomultiplier. A combination of band-reject filters attenuates the 980 nm laser excitation light and its harmonic at 490 nm, and a tunable liquid crystal filter provides for rapid scanning from 400 to 750 nm. The data acquisition and control is controlled by a laptop PC with a custom GUI interface developed using LabWindows/CVI. The system can detect a single phosphor particle bound to a capture surface.

Paper Details

Date Published: 22 May 1997
PDF: 7 pages
Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); doi: 10.1117/12.274359
Show Author Affiliations
Haris Riris, SRI International (United States)
Jan E. Van der Laan, SRI International (United States)
David E. Cooper, SRI International (United States)
Karen M. Nashold, SRI International (United States)
Clinton B. Carlisle, SRI International (United States)
Luke V. Schneider, SRI International (United States)

Published in SPIE Proceedings Vol. 2985:
Ultrasensitive Biochemical Diagnostics II
Gerald E. Cohn; Gerald E. Cohn; Steven A. Soper, Editor(s)

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