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Proceedings Paper

Prototype fluorimeter for the XLA/XLI analytical ultracentrifuge
Author(s): Thomas M. Laue; Arthur L. Anderson; Bryan J. Weber
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Paper Abstract

A fluorescence detector has been developed for the XLA/XLI analytical ultracentrifuge. The excitation source consists of an Argon ion laser coupled to a single-mode, 3.5 micron diameter fiber. The remaining optics are in the rotor chamber and attached to a 500 steps/second, 2 microns/step motorized stage mounted above the centrifuge rotor. The excitation and emission paths are coaxial. The excitation spot is about 7 microns in diameter and is focused about 1 mm below the top window of a centrifuge cell. Radial resolution is achieved by scanning this spot along a radial axis. Emitted light is gathered by the condensing lens and redirected by the beam splitter to a condensing lens focused on a pinhole. A long-pass filter sits between the pinhole and a photomultiplier tube. Operation of the stepping motor and photomultiplier electronics is under computer control, allowing data to be acquired from a radial stripe around the rotor after each step. This data acquisition system will permit the monitoring of specifically labeled compounds in the presence of a large background of other molecules. Both the sensitivity and the selectivity of this optical system will be useful for a range of biological problems.

Paper Details

Date Published: 22 May 1997
PDF: 9 pages
Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); doi: 10.1117/12.274351
Show Author Affiliations
Thomas M. Laue, Univ. of New Hampshire (United States)
Arthur L. Anderson, Univ. of New Hampshire (United States)
Bryan J. Weber, Beckman Instruments, Inc. (United States)

Published in SPIE Proceedings Vol. 2985:
Ultrasensitive Biochemical Diagnostics II
Gerald E. Cohn; Gerald E. Cohn; Steven A. Soper, Editor(s)

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