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Proceedings Paper

Detection and analysis of polymerase chain reaction products by mass spectrometry
Author(s): Gregory B. Hurst; Mitchel J. Doktycz; Philip F. Britt; Arpad A. Vass; Michelle V. Buchanan
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Paper Abstract

While molecular weight determination of proteins by matrix- assisted laser desorption/ionization (MALDI) has been quite successful, the measurement of deoxyribonucleic acid polymers in a size range useful for fine-structure genome analysis has proven more difficult, due to decreasing sensitivity and resolution at the higher molecular weights. Tailored cleanup techniques and the use of delayed extraction time-of-flight mass spectrometry are approaches that offer considerable promise for improved MALDI analysis of DNA.

Paper Details

Date Published: 22 May 1997
PDF: 9 pages
Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); doi: 10.1117/12.274345
Show Author Affiliations
Gregory B. Hurst, Oak Ridge National Lab. (United States)
Mitchel J. Doktycz, Oak Ridge National Lab./Health Sciences Research Div. (United States)
Philip F. Britt, Oak Ridge National Lab. (United States)
Arpad A. Vass, Oak Ridge National Lab./Health Sciences Research Div. (United States)
Michelle V. Buchanan, Oak Ridge National Lab. (United States)


Published in SPIE Proceedings Vol. 2985:
Ultrasensitive Biochemical Diagnostics II
Gerald E. Cohn; Gerald E. Cohn; Steven A. Soper, Editor(s)

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