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Proceedings Paper

Diode-laser-based time-resolved detection and identification of individual mononucleotide molecules in aqueous solution
Author(s): Markus Sauer; Christoph Zander; Ralph Mueller; Florian Goebel; Andreas Schulz; Stefan Siebert; Karl-Heinz Drexhage; Juergen M. Wolfrum
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Paper Abstract

We applied a short-pulse diode laser emitting at 637 nm with a repetition rate of 30 MHz in combination with a confocal microscope to study bursts of fluorescence photons from individual labeled mononucleotide molecules in water. A newly synthesized oxazine dye and the commercially available carbocyanine dye Cy5 were used as fluorescent labels. Multichannel scalar traces, the fluorescence autocorrelation function and fluorescence decay times determined by time- correlated single-photon counting have been measured simultaneously. The time-resolved signals of the two mononucleotides were analyzed and identified by a maximum likelihood estimator. The results showed out that 60 detected photons per transit of a single molecule are sufficient to distinguish two labeled mononucleotides in water with a misclassification of less than 10 percent via their characteristic fluorescence lifetimes of 1.07 +/- 0.27 ns and 1.89 +/- 0.34 ns.

Paper Details

Date Published: 22 May 1997
PDF: 8 pages
Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); doi: 10.1117/12.274338
Show Author Affiliations
Markus Sauer, Univ. Heidelberg (Germany)
Christoph Zander, Univ.-Gesamthochschule Siegen (Germany)
Ralph Mueller, Univ. Heidelberg (Germany)
Florian Goebel, Univ. Heidelberg (Germany)
Andreas Schulz, Univ. Heidelberg (Germany)
Stefan Siebert, Univ. Heidelberg (Germany)
Karl-Heinz Drexhage, Univ.-Gesamthochschule Siegen (Germany)
Juergen M. Wolfrum, Ruprecht-Karls-Univ. Heidelberg (Germany)

Published in SPIE Proceedings Vol. 2985:
Ultrasensitive Biochemical Diagnostics II
Gerald E. Cohn; Gerald E. Cohn; Steven A. Soper, Editor(s)

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