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Proceedings Paper

Cell damage in UVA and cw/femtosecond NIR microscopes
Author(s): Karsten Koenig; Hong Liang; Sol Kimel; Lars Othar Svaasand; Bruce J. Tromberg; Tatiana B. Krasieva; Michael W. Berns; Karl-Juergen Halbhuber; Peter T. C. So; William W. Mantulin; Enrico Gratton
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Paper Abstract

Cell damage in UV and NIR laser microscopes by highly focused micromanipulation and fluorescence excitation microbeams has been studied. Damage in erythrocytes, spermatozoa and Chinese hamster ovary cells was detected by monitoring morphology changes, autofluorescence detection, cloning assay, and viability screening. It was found that 364 nm/365 nm UVA radiation induced irreversible cell damage at radiant exposures as low as <10 J/cm2. NIR CW microradiation used in laser tweezers was also able to damage cells via a two-photon excitation process, in particular, when using <800 nm trapping beams. Non- destructive two-photon excitation in femtosecond NIR microscopes is possible within a narrow intensity window. The lower limit is determined by two-photon absorption coefficients and detector efficiency, the higher by intracellular optical breakdown in the extranuclear region. Above certain wavelength-dependent intensity thresholds in femtosecond microscopy, cells were completely destroyed by fragmentation concomitant with plasma generation. The influence of excitation and micromanipulation microbeams should be considered when studying physiology and metabolism of vital cells.

Paper Details

Date Published: 23 May 1997
PDF: 8 pages
Proc. SPIE 2983, Functional Imaging and Optical Manipulation of Living Cells, (23 May 1997); doi: 10.1117/12.274337
Show Author Affiliations
Karsten Koenig, Friedrich Schiller Univ. Jena (Germany)
Hong Liang, Beckman Laser Institute and Medical Clinic (United States)
Sol Kimel, Technion--Israel Institute of Technology (Israel)
Lars Othar Svaasand, Univ. of Trondheim (Norway)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)
Karl-Juergen Halbhuber, Friedrich Schiller Univ. Jena (Germany)
Peter T. C. So, Univ. of Illinois/Urbana-Champaign (United States)
William W. Mantulin, Univ. of Illinois/Urbana-Champaign (United States)
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 2983:
Functional Imaging and Optical Manipulation of Living Cells
Daniel L. Farkas; Bruce J. Tromberg, Editor(s)

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