Share Email Print
cover

Proceedings Paper

Laser microbeam abalation of GFP-labeled nuclear organelles in a living cell.
Author(s): Vickie J. LaMorte; Tatiana B. Krasieva; Ronald M. Evans; Michael W. Berns; Bruce J. Tromberg
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Cancer, development, cellular growth and differentiation are governed by gene expression. Recent molecular and cellular advances to visualize and perturb the pathways of transcriptional regulation, nascent RNA processing, and protein trafficking at the single cell level have been developed. More recently, applications utilizing the green fluorescent marker (GFP) from Aequorea victoria have facilitated visualization of these molecular events in a living cell. Specifically, we will describe a novel approach to perturb cellular processes by labeling discrete cellular components of interest with GFP and subsequently altering/ablating them with a laser microbeam.

Paper Details

Date Published: 23 May 1997
PDF: 5 pages
Proc. SPIE 2983, Functional Imaging and Optical Manipulation of Living Cells, (23 May 1997); doi: 10.1117/12.274333
Show Author Affiliations
Vickie J. LaMorte, Beckman Laser Institute and Medical Clinic (United States)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic (United States)
Ronald M. Evans, Howard Hughes Medical Institute (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 2983:
Functional Imaging and Optical Manipulation of Living Cells
Daniel L. Farkas; Bruce J. Tromberg, Editor(s)

© SPIE. Terms of Use
Back to Top