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Proceedings Paper

X-ray imaging of living cells
Author(s): Toshikazu Majima; Hideaki Shimizu; Toshihisa Tomie; Eisuke Miura; Toshihiko Kanayama; Masahiro Yamada
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Paper Abstract

X-ray images of living cells were obtained by using a flash contact x-ray microscope system. The system consists of a high power laser (grass laser or KrF laser) for producing a plasma as an x-ray source, a small vacuum chamber, and a sample holder with a metal target for making plasma. The x- ray images were recorded on an x-ray sensitive layer on a silicon wafer. After chemical development x-ray images on the x-ray sensitive layers were read out by using an atomic force microscope. The performance of the system is demonstrated by presenting x-ray images of unicellular microorganisms, bacteria and skeletal muscle fibers. The intracellular structures were visible in some cases. For laboratory use a flash contact table-top x-ray microscopy is improved using a table-scaled YAG laser. The table-top systems showed good performance comparing to that of the x- ray microscope system with high power laser. These results indicate that the table-top x-ray microscope is a powerful instrumentation for the observation of living cells.

Paper Details

Date Published: 23 May 1997
PDF: 8 pages
Proc. SPIE 2983, Functional Imaging and Optical Manipulation of Living Cells, (23 May 1997); doi: 10.1117/12.274326
Show Author Affiliations
Toshikazu Majima, Electrotechnical Lab. (Japan)
Hideaki Shimizu, Electrotechnical Lab. (Japan)
Toshihisa Tomie, Electrotechnical Lab. (Japan)
Eisuke Miura, Electrotechnical Lab. (Japan)
Toshihiko Kanayama, Electrotechnical Lab. (Japan)
Masahiro Yamada, Electrotechnical Lab. (Japan)

Published in SPIE Proceedings Vol. 2983:
Functional Imaging and Optical Manipulation of Living Cells
Daniel L. Farkas; Bruce J. Tromberg, Editor(s)

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