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Proceedings Paper

Digital measurement of gene expression in a cDNA microarray
Author(s): Edward R. Dougherty; Yidong Chen; Sinan Batman; Michael L. Bittner
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Paper Abstract

Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA micro- array. Comparison of expression levels arising from co- hybridized samples is achieved by taking ratios of average expression levels for individual genes. The present paper concerns image processing to automatically segment digitized micro-arrays and measure median gene expression levees across cDNA target sites. The main difficulty arises from determination of the target site when signal intensity is low. Segmentation must be accomplished for target sites that can possess highly unstable geometry and consist of a relatively small number of pixels. Segmentation must also be computationally efficient. The present paper proposes a nonparametric statistical method that separates target site from local background using the Mann-Whitney test.

Paper Details

Date Published: 25 April 1997
PDF: 6 pages
Proc. SPIE 3034, Medical Imaging 1997: Image Processing, (25 April 1997); doi: 10.1117/12.274178
Show Author Affiliations
Edward R. Dougherty, Texas A&M Univ. (United States)
Yidong Chen, National Institutes of Health (United States)
Sinan Batman, Texas A&M Univ. (United States)
Michael L. Bittner, National Institutes of Health (United States)


Published in SPIE Proceedings Vol. 3034:
Medical Imaging 1997: Image Processing
Kenneth M. Hanson, Editor(s)

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