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Proceedings Paper

Temperature dependence of erythrocyte aggregation in vitro by backscattering nephelometry
Author(s): Igor V. Sirko; Nikolai N. Firsov; Olga M. Ryaboshapka; Alexander V. Priezzhev
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Paper Abstract

We apply backscattering nephelometry technique to register the alterations of the scattering signal from a whole blood sample due to appearance or disappearance of different types of erythrocyte aggregates in stasis and under controlled shear stress. The measured parameters are: the characteristic times of linear and 3D aggregates formation, and the strength of aggregates of different types. These parameters depend on the sample temperature in the range of 2 divided by 50 degrees C. Temporal parameters of the aggregation process strongly increase at temperature 45 degrees C. For samples of normal blood the aggregates strength parameters do not significantly depend on the sample temperature, whereas for blood samples from patients suffering Sjogren syndrome we observe high increase of the strength of 3D and linear aggregates and decrease of time of linear aggregates formation at low temperature of the sample. This combination of parameters is opposite to that observed in the samples of pathological blood at room temperature. Possible reasons of this behavior of aggregation state of blood and explanation of the observed effects will be discussed.

Paper Details

Date Published: 2 May 1997
PDF: 5 pages
Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); doi: 10.1117/12.273630
Show Author Affiliations
Igor V. Sirko, Russian State Medical Univ. (Russia)
Nikolai N. Firsov, Russian State Medical Univ. (Russia)
Olga M. Ryaboshapka, Moscow State Univ. (Russia)
Alexander V. Priezzhev, Moscow State Univ. (Russia)

Published in SPIE Proceedings Vol. 2982:
Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology
Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Robert C. Leif, Editor(s)

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