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Proceedings Paper

Refractive-index-based calorimetric studies of RNAse T1 unfolding in small volumes using microinterferometric backscatter
Author(s): Michael P. Houlne; Darren S. Hubbard; George I. Makhatadze; Darryl J. Bornhop
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Paper Abstract

Micro-interferometry, a novel technique developed by the authors, employs a linearly polarized laser, a fused silica capillary tube housing for the sample and a charged coupled device as a detector. A back scattered interference pattern, observed as a high contrast fringes, is produced when the laser is directed onto the capillary containing the sample. The positional change of the fringe pattern is a function of the refractive index of the media in the capillary. In the present work, the RNA enzyme RNase T1 is heated in the sample cell over a temperature range of 30 degrees C to 60 degrees C. Over this temperature range the molecule unfolds form the quaternary to the tertiary structure. This structure change is manifested as a refractive index change and is observed by monitoring the fringe position while ramping the cell temperature in a controlled fashion. From the refractive index response over the temperature range, the Gibbs free energy associated with unfolding is calculated. The authors show milli-degree temperature stability with a 0.1 micro-liter probe volume, thus demonstrating the application of this device in micro- calorimetric investigations.

Paper Details

Date Published: 2 May 1997
PDF: 9 pages
Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); doi: 10.1117/12.273610
Show Author Affiliations
Michael P. Houlne, Texas Tech Univ. (United States)
Darren S. Hubbard, Texas Tech Univ. (United States)
George I. Makhatadze, Texas Tech Univ. (United States)
Darryl J. Bornhop, Texas Tech Univ. and Southwest Cancer Ctr. (United States)


Published in SPIE Proceedings Vol. 2982:
Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology
Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Robert C. Leif, Editor(s)

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