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Proceedings Paper

Modeling single-molecule detection statistics
Author(s): Joerg Enderlein; David L. Robbins; W. Patrick Ambrose; Peter M. Goodwin; Richard A. Keller
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Paper Abstract

We present experimental results of single B-phycoerythrin molecule detection in a fluid flow at different sample introduction rates. A new mathematical approach is used for calculating the resulting burst size distributions. The calculations are based upon a complete physical model including absorption, fluorescence and photobleaching characteristics of the fluorophore; its diffusion; the sample stream hydrodynamics; the spatially dependent optical detection efficiency; and the excitation laser beam characteristics. Special attention is paid to the phenomenon of `molecular noise'--fluctuations in the number of overlapping crossings of molecules through the detection volume. The importance of this study and its connections to experimental applications are discussed.

Paper Details

Date Published: 7 May 1997
PDF: 12 pages
Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273570
Show Author Affiliations
Joerg Enderlein, Los Alamos National Lab. (United States)
David L. Robbins, Los Alamos National Lab. (United States)
W. Patrick Ambrose, Los Alamos National Lab. (United States)
Peter M. Goodwin, Los Alamos National Lab. (United States)
Richard A. Keller, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 2980:
Advances in Fluorescence Sensing Technology III
Richard B. Thompson, Editor(s)

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