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Proceedings Paper

Lifetime identification of single molecules in aqueous solution
Author(s): Christoph Zander; Markus Sauer; Karl-Heinz Drexhage; Juergen M. Wolfrum; Leif Brand; C. Eggeling; Claus A. M. Seidel
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Paper Abstract

Photon bursts of single rhodamine 6G and rhodamine B molecules in aqueous solution were studied by excitation with a frequency-doubled titanium: sapphire laser. Multichannel scalar traces, fluorescence correlation functions and fluorescence decays determined by time- correlated single-photon counting have been measured simultaneously. The time-resolved fluorescence signals were analyzed with a maximum likelihood estimator. With the setup described it is possible to distinguish single dye molecules of different kind via their characteristic fluorescence lifetimes of 1.79 +/- 0.33 ns for rhodamine B-zwitterion and 3.79 +/- 0.38 ns for rhodamine 6G.

Paper Details

Date Published: 7 May 1997
PDF: 8 pages
Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273566
Show Author Affiliations
Christoph Zander, Univ.-Gesamthochschule Siegen (Germany)
Markus Sauer, Univ. Heidelberg (Germany)
Karl-Heinz Drexhage, Univ.-Gesamthochschule Siegen (Germany)
Juergen M. Wolfrum, Ruprecht-Karls-Univ. Heidelberg (Germany)
Leif Brand, Max-Planck-Institut fuer Biophysikalische Chemie (Germany)
C. Eggeling, Max-Planck-Institut fuer Biophysikalische Chemie (Germany)
Claus A. M. Seidel, Max-Planck-Institut fuer Biophysikalische Chemie (Germany)


Published in SPIE Proceedings Vol. 2980:
Advances in Fluorescence Sensing Technology III
Richard B. Thompson, Editor(s)

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