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Proceedings Paper

Two-photon fluorescence of DNA-dye complexes
Author(s): Lia A. Avanessian; Vladimir A. Hovanessian
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Paper Abstract

Preliminary results of the investigation of two-photon excited fluorescence of acridine orange (AO) and ethidium bromide (EB) in complexes with DNA are presented. Spectrofluorometer based on picosecond Nd:YAG laser was used for investigations on two-photon (1064 nm, 1 mJ, 40 ps) and one-photon (532 and 355 nm) excitation. Highly purified DNA from beef spleen and AO, EB were used at the concentration of: AO- 15 (mu) M, EB- 12 (mu) M, DNA- 2-15 (mu) g/ml. The spectra of two-photon excited fluorescence of AO, EB and their complexes with DNA as well as kinetics of the intensification of dye's two-photon fluorescence during their interactions with DNA in dependence on DNA concentration were obtained. The intensity of fluorescence of AO and EB was increased maximum 2.4 and 8 times correspondingly. During the one-photon excitation corresponding values were for: AO-2.5 time and EB-10 time. The difference in the intensification of EB fluorescence connected with the difference of the change of one-photon and two-photon absorption coefficients during the interaction of the dye with DNA.

Paper Details

Date Published: 7 May 1997
PDF: 5 pages
Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273565
Show Author Affiliations
Lia A. Avanessian, Institute of Epidemiology, Virology and Medical Parisitology (Armenia)
Vladimir A. Hovanessian, Yerevan State Univ. (Armenia)

Published in SPIE Proceedings Vol. 2980:
Advances in Fluorescence Sensing Technology III
Richard B. Thompson, Editor(s)

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