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Proceedings Paper

Fluorescent characteristics and pharmacokinetic profiles of the fluorescent probe BCECF in various tissues: the role of blood content
Author(s): Jean-Marie Devoisselle; Sylvie Soulie-Begu; Serge R. Mordon; H. Maillols
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Paper Abstract

Microspectrofluorometry and fluorescence imaging have been largely used to measure the intracellular pH of living cells. Such in vivo measurements require the knowledge of the optical properties of the tissue and the pharmacokinetic of the dye. The purpose of this study was to investigate in vitro and in vivo spectral characteristics of BCECF. In vitro, measurements in presence of blood show that blood content can greatly affect pH measurement by increasing the fluorescence ratio. In vivo, the presence of blood in high vascularized tissue leads to the modification of BCECF spectral characteristics. Fluorescence kinetic profiles provides information about tissue perfusion. Consequently, pH measurements using BCECF or fluorescein derivatives by the double excitation method may be performed taking in account the tissue blood content and the tissue pharmacokinetic of the dye.

Paper Details

Date Published: 7 May 1997
PDF: 11 pages
Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273547
Show Author Affiliations
Jean-Marie Devoisselle, Univ. de Montpellier I (France)
Sylvie Soulie-Begu, Univ. de Montpellier I (France)
Serge R. Mordon, INSERM (France)
H. Maillols, Univ. de Montpellier I (France)


Published in SPIE Proceedings Vol. 2980:
Advances in Fluorescence Sensing Technology III
Richard B. Thompson, Editor(s)

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