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Proceedings Paper

Fluorescence detection of the anticancer drug topotecan and related camptothecins in plasma and whole blood by two-photon excitation
Author(s): Thomas G. Burke; Henryk M. Malak; Ignacy Gryczynski; Joseph R. Lakowicz
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Paper Abstract

Recent FDA-approval of topotecan (9-dimethylaminomethyl-10- hydroxycamptothecin) and camptosar (CPT-11) along with the accelerated clinical development of related camptothecin drugs provides new hope for the successful treatment of human cancer, including neoplasms for which no effective treatments currently exist. Current clinical efforts worldwide are aimed at optimizing the therapeutic efficacies of the camptothecins, with the major focus on the determination of the most effective dosing schedules. To this end, technological advances which provide a direct and rapid means of measuring plasma drug levels (i.e. such that correlations between plasma drug levels and clinical responses can be sought) would be of great utility. Here we report on the direct fluorescence detection of topotecan and SN-38 in human plasma and topotecan in whole blood at micro molar levels using two-photon excitation at 730 or 820 nm. Topotecan was detected at concentrations as low as 0.05 and 1 (mu) M in plasma and whole blood, respectively. Since skin, blood and other tissues are translucent at long wavelengths, our results suggest the attractive possibility of homogeneous or noninvasive clinical sensing of camptothecins in situ using two-photon excitation.

Paper Details

Date Published: 7 May 1997
PDF: 11 pages
Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273532
Show Author Affiliations
Thomas G. Burke, Univ. of Kentucky College of Pharmacy and Markey Cancer Ctr. and Ohio State Univ. College (United States)
Henryk M. Malak, Univ. of Maryland School of Medicine/Baltimore (United States)
Ignacy Gryczynski, Univ. of Maryland School of Medicine/Baltimore (United States)
Joseph R. Lakowicz, Univ. of Maryland School of Medicine/Baltimore (United States)

Published in SPIE Proceedings Vol. 2980:
Advances in Fluorescence Sensing Technology III
Richard B. Thompson, Editor(s)

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