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Proceedings Paper

Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier
Author(s): Keith Dowling; Sam C. W. Hyde; Nicholas P. Barry; Christopher Dainty; Paul M. W. French; Alun J. Hughes; M. John Lever; Anthony K. L. Dymoke-Bradshaw; Jonathan D. Hares; Paul A. Kellett
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Paper Abstract

We report the demonstration of a high temporal resolution fluorescence lifetime imaging (FLIM) system using a time- gated image intensifier to provide whole field FLIM images. The gate width has been optimized to 110 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences of 20 ps, have been detected. Environmental changes of the fluorescent indicator, Lucifer Yellow, have been sensed by measuring changes in its fluorescence lifetime when unbound and when bound to the protein albumin.

Paper Details

Date Published: 7 May 1997
PDF: 4 pages
Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); doi: 10.1117/12.273522
Show Author Affiliations
Keith Dowling, Imperial College of Science, Technology and Medicine (United Kingdom)
Sam C. W. Hyde, Imperial College of Science, Technology and Medicine (United Kingdom)
Nicholas P. Barry, Imperial College of Science, Technology and Medicine (United Kingdom)
Christopher Dainty, Imperial College of Science, Technology and Medicine (United Kingdom)
Paul M. W. French, Imperial College of Science, Technology and Medicine (United Kingdom)
Alun J. Hughes, Imperial College of Science, Technology and Medicine (United Kingdom)
M. John Lever, Imperial College of Science, Technology and Medicine (United Kingdom)
Anthony K. L. Dymoke-Bradshaw, Kentech Instruments Ltd. (United Kingdom)
Jonathan D. Hares, Kentech Instruments Ltd. (United Kingdom)
Paul A. Kellett, Kentech Instruments Ltd. (United Kingdom)


Published in SPIE Proceedings Vol. 2980:
Advances in Fluorescence Sensing Technology III
Richard B. Thompson, Editor(s)

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