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Proceedings Paper

Increase in reactive oxygen species production and phagocytic activity of polymorphonuclear neutrophils stimulated by preirradiated hematoporphyrin-derivative solution
Author(s): Vladislava Melnikova; Lina N. Bezdetnaya; Irina Belitchenko; Alla A. Kyagova; Pascal Colosetti; Alexander Ya. Potapenko; Francois H. Guillemin
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Paper Abstract

We have studied the influence of preirradiated by visible light hematoporphyrin derivative (HpD) solution in PBS on the production of reactive oxygen species (ROS) and phagocytosis of latex particles by rat peritoneal polymorphonuclear neutrophils (PMN), and also on the delayed type hypersensitivity reaction (DTH) to sheep red blood cells in mice. The release of ROS and phagocytic activity were observed by means of registration of the luminol- enhanced chemiluminescence (ChL) in the absence and the in the presence of latex particles. Non-irradiated HpD did not influence neither spontaneous ChL response, nor latex- activated. HpD preirradiated by 135 J/m2 did not affect spontaneous, but increased latex-activated ChL response by 20 percent. This fact indicates an increase in PMN phagocytic activity under the treatment with preirradiated HpD. Increase in preirradiation fluence up to 8.1 kJ/m2 resulted in significant enhancement of spontaneous ChL and inhibition of latex-activated ChL response of PMN. Results of spectroscopic analysis showed negligible decease in HpD Soret band after preirradiation of HpD by the highest fluences used in this study. We could not detect any significant photoproduct formation by differential absorption spectroscopy. Earlier, we have propose the photoinduced aggragation as one of the possible mechanisms of photodegradation of aqueous porphyrin solutions. In all probability, the increase in ROS production by PMN, treated with preirradiated HpD can be attributed to the phagocytosis of aggregates formed. It is possible that ROS can influence directly the DTH-effector cells leading to the observed decrease in DTH reaction level.

Paper Details

Date Published: 8 May 1997
PDF: 7 pages
Proc. SPIE 2972, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VI, (8 May 1997); doi: 10.1117/12.273496
Show Author Affiliations
Vladislava Melnikova, Ctr. Alexis Vautrin (France) and Russian State Medical Univ. (Russia)
Lina N. Bezdetnaya, Ctr. Alexis Vautrin (Russia)
Irina Belitchenko, Ctr. Alexis Vautrin (Russia)
Alla A. Kyagova, Russian State Medical Univ. (Russia)
Pascal Colosetti, Ctr. Alexis Vautrin (France)
Alexander Ya. Potapenko, Russian State Medical Univ. (Russia)
Francois H. Guillemin, Ctr. Alexis Vautrin (France)

Published in SPIE Proceedings Vol. 2972:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VI
Thomas J. Dougherty, Editor(s)

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