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Proceedings Paper

Oriented bacteriorhodopsin film biomolecular devices and their photoelectric dynamics
Author(s): Baoli Yao; Dalun Xu; Xun Hou
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Paper Abstract

Thin oriented bacteriorhodopsin films isolated from the Halobacterium halobium R1M1 strain are made on transparent conductive ITO glass and stainless sheet respectively by the use of electrophoretic sedimentation method and Langmuir-Blodgett technique. To construct photoelectric devices, another Cu or Al electrode is applied, and electrolytes or insulant films are sandwiched between the electrodes. A Q-switched double frequency Nd:YAG laser and a pulsed dye laser are used to stimulate the bacteriorhodopsin films. The photoelectric response signals are directly measured by a 300 MHz digitizing oscilloscope. Experimental curves are fitted through the use of Marquardt's algorithm least square method, and at least five exponential decay components are found in the whole process. A theory on the bacteriorhodopsin film photoelectric dynamics is proposed and the dynamic equations are mathematically derived. Comparison between the theoretical and experimental results gives the parameters of time constants and charge displacements in the bacteriorhodopsin photocycle.

Paper Details

Date Published: 28 May 1997
PDF: 7 pages
Proc. SPIE 2869, 22nd International Congress on High-Speed Photography and Photonics, (28 May 1997); doi: 10.1117/12.273484
Show Author Affiliations
Baoli Yao, Xi'an Institute of Optics and Precision Mechanics (China)
Dalun Xu, Xi'an Institute of Optics and Precision Mechanics (China)
Xun Hou, Xi'an Institute of Optics and Precision Mechanics (China)


Published in SPIE Proceedings Vol. 2869:
22nd International Congress on High-Speed Photography and Photonics
Dennis L. Paisley; ALan M. Frank, Editor(s)

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