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Proceedings Paper

Improved resolution in PSAF imaging
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Paper Abstract

In PSAF (point spread autocorrelation function) imaging a fluorescence signal is generated from the interference response in the overlap region of two spatially shifted point spread functions (PSFs). It is experimentally demonstrated that a resolution improvement of approximately 30% can be realized in the case of axially shifted PSFs under high numerical-aperture (NA) conditions. A similar improvement in resolution is expected from numerical modeling for the lateral case. The presented technique can also be applied to the measurement of the effective point spread function itself in all three dimensions. It is found that the technique -- which in the latter case uses a bulk fluorescing solution -- is an excellent tool to access the apodization conditions of a practical optical system, such as a high-numerical aperture objective.

Paper Details

Date Published: 10 April 1997
PDF: 9 pages
Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); doi: 10.1117/12.271269
Show Author Affiliations
Michiel Mueller, Univ. of Amsterdam (Netherlands)
G. J. Brakenhoff, Univ. of Amsterdam (Netherlands)


Published in SPIE Proceedings Vol. 2984:
Three-Dimensional Microscopy: Image Acquisition and Processing IV
Carol J. Cogswell; Jose-Angel Conchello; Tony Wilson, Editor(s)

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