Share Email Print
cover

Proceedings Paper

Virus structure using the computer-aided phase microscope Airyscan
Author(s): Vladimir P. Tychinsky; Nikolai V. Kaverin; Elena V. Perevedentseva; Tatiana V. Vyshenskaia; Georgy E. Kufal
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Investigation of features and functions of some small biological objects (smaller than 500 nm), in particular, viruses, with conventional optical microscopy is practically impossible. Usually their images are obtained with methods of scanning electron microscopy (SEM), which precludes work with samples in a native state. We obtained images of different viruses including influenza A virus in native state with computer-aided phase microscope (CPM) Airyscan, in which an He-Ne laser is used as a light source. The main purpose of this work was to show the possibility to obtain adequate structure images of influenza viruses with diameter about 100 nm in conditions quite close to native and to investigate different stages of influenza virus budding. We suppose that these results may be considered as a basis for further studies of cell-virus interaction.

Paper Details

Date Published: 10 April 1997
PDF: 7 pages
Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); doi: 10.1117/12.271268
Show Author Affiliations
Vladimir P. Tychinsky, Moscow Institute for Radioengineering, Electronics and Automation (Russia)
Nikolai V. Kaverin, D. I. Ivanovsky Institute of Virology (Russia)
Elena V. Perevedentseva, Moscow Institute for Radioengineering, Electronics and Automation (Russia)
Tatiana V. Vyshenskaia, Moscow Institute for Radioengineering, Electronics and Automation (Russia)
Georgy E. Kufal, Moscow Institute for Radioengineering, Electronics and Automation (Russia)


Published in SPIE Proceedings Vol. 2984:
Three-Dimensional Microscopy: Image Acquisition and Processing IV
Carol J. Cogswell; Jose-Angel Conchello; Tony Wilson, Editor(s)

© SPIE. Terms of Use
Back to Top