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Proceedings Paper

Three-dimensional imaging through millimeter-thick tissue specimens
Author(s): Michael G. Jones; Michael Halliwell; David R. Bull; Jack D. Davies; Catherine N. Chinyama; Peter N. T. Wells
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Paper Abstract

A 780 nm stage-scanned confocal transmission microscope using compact disk (CD) player optics has been constructed and used to study test objects (including scattering effects) and breast tissue specimens (25-1500 micrometer thick). Confocal alignment in transmission is achieved using the CD optical mechanism moving coils to scan the objective lens until intensity peaks; the specimen is present but motionless. Intensity variation as a 3D function of mis- alignment of the two foci may allow estimation of optic deterioration caused by the specimen; appropriate confocal apertures and sample spacings can then be selected. Experimental measurements have been supplemented by 2D Monte-Carlo modeling of photon transport for a scattering- limited confocal transmission system. Modeling has characterized: transmission as a function of focal depth in a slab; contrast produced by a range of embedded spherical target radii; and optical mis-alignment. Poor dye perfusion within thick stained specimens results in little visible internal structure. This may be improved using partially phase dependent imaging (approximately equal to split- detector), sensitive to specimen refractive index variations.

Paper Details

Date Published: 10 April 1997
PDF: 12 pages
Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); doi: 10.1117/12.271263
Show Author Affiliations
Michael G. Jones, United Bristol & NHS Trust & Bristol General Hospital (United Kingdom)
Michael Halliwell, United Bristol & NHS Trust & Bristol General Hospital (United Kingdom)
David R. Bull, Univ. of Bristol (United Kingdom)
Jack D. Davies, Univ. of Bristol and Southmead Hospital (United Kingdom)
Catherine N. Chinyama, Univ. of Bristol and Southmead Hospital (United Kingdom)
Peter N. T. Wells, Univ. of Bristol and NHS Trust (United Kingdom)


Published in SPIE Proceedings Vol. 2984:
Three-Dimensional Microscopy: Image Acquisition and Processing IV
Carol J. Cogswell; Jose-Angel Conchello; Tony Wilson, Editor(s)

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