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Proceedings Paper

Cell attachment on microscopically textured silicon surfaces
Author(s): Stephen W. Turner; Lance Kam; Michael Isaacson; Harold G. Craighead; Donald H. Szarowski; James N. Turner; W. Shain
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Paper Abstract

To assess the effect of surface topography on cell attachment, central nervous system (astroglial cells) cells were grown on surfaces patterned with two different types of texture. Reactive ion etching (RIE) was used to induce nanometer-scale roughness in silicon wafers. In a subsequent wet etch, photo-patterned resist protected selected areas of the surface, resulting in a pattern of modified and unmodified texture. Scanning electron microscopy (SEM) showed that the RIE-roughened 'primary' surface consists of randomly positioned columnar structures (diameter approximately equals 50 nm, height approximately equals 250 nm). The wet-etched 'secondary' surfaces had shorter and more sparsely distributed projections, controlled to a degree by wet etch duration. Confocal microscopy and SEM demonstrated that transformed astroglial (LRM55) cells preferred secondary surfaces. The morphology of cells on secondary surfaces depended on wet etch duration. with brief wet etch, cells hade stellate or mounded morphology and were not closely adherent to the surface. With long wet etch, cells had an epithelial-like morphology and were closely adherent to substrates. Under all conditions, cells discriminated between primary and secondary surfaces. In contrast to LRM55 cells, astrocytes in primary cell culture preferred primary surfaces. Thus changes in surface topography produce cell-specific selectivity and change cell attachment characteristics.

Paper Details

Date Published: 31 March 1997
PDF: 12 pages
Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); doi: 10.1117/12.269978
Show Author Affiliations
Stephen W. Turner, Cornell Univ. (United States)
Lance Kam, New York State Dept. of Health, SUNY/Albany, and Rensselaer Polytechnic Institute (United States)
Michael Isaacson, Cornell Univ. (United States)
Harold G. Craighead, Cornell Univ. (United States)
Donald H. Szarowski, New York State Dept. of Health and SUNY/Albany (United States)
James N. Turner, New York State Dept. of Health and SUNY/Albany (United States)
W. Shain, New York State Dept. of Health and SUNY/Albany (United States)


Published in SPIE Proceedings Vol. 2978:
Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications
Paul Lee Gourley, Editor(s)

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