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Proceedings Paper

Development and comparison of pH microoptodes for use in marine systems
Author(s): Oliver Kohls; Ingo Klimant; Gerhard A. Holst; Michael Kuehl
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Paper Abstract

Traditionally microscale measurements of pH are based on potentiometric measurements with a pH glass microelectrode. The preparation of these electrodes is, however, very time consuming. We developed pH micro-optodes for use in seawater in the range of pH 7 - 9. The optodes are based on immobilized acid-base indicators, which change their color and/or fluorescence properties as a function of the pH. Various dyes were immobilized directly on the tip of a tapered optical fiber by different techniques. We then investigated these pH optodes with respect to response time, mechanical stability and calibration characteristics. Dependent on the optical properties of the indicator material we used different light emitting diodes (LEDs) as the light sources and either a photodiode or a photomultiplier as detector.

Paper Details

Date Published: 31 March 1997
PDF: 10 pages
Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); doi: 10.1117/12.269958
Show Author Affiliations
Oliver Kohls, Max-Planck-Institute for Marine Microbiology (Germany)
Ingo Klimant, Univ. Regensburg (Germany)
Gerhard A. Holst, Max-Planck-Institute for Marine Microbiology (Germany)
Michael Kuehl, Max-Planck-Institute for Marine Microbiology (Germany)


Published in SPIE Proceedings Vol. 2978:
Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications
Paul Lee Gourley, Editor(s)

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