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Proceedings Paper

Simple method of enzyme immobilization for pH-ISFET-based urea biosensors
Author(s): Dorota Pijanowska; Wladislaw Torbicz
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Paper Abstract

In this paper, a simple chemical method of urease immobilization on silicon nitride surface is described. As a basic structure to construct urea-biosensor, a pH-sensitive Si3N4-gate ISFET was used. The developed method of chemical immobilization of urease is based on Schiff's base formation. The developed EnFET type urea biosensor are characterized by the following parameters: (1) maximum analytical signal: 120 divided by 140 mV in 10 mM phosphate buffer solution, (2) linear range of the (Delta) Ugs equals f(logCurea): pCurea(2 divided by 3.5) in 10 mM phosphate buffer, (3) response time: 80 s and (4) lifetime: 35 days with the stable analytical signal then after 52 days this signal decreased by at least 40%. The influence of the concentration as well as the pH of the buffer solution on EnFET response were investigated.

Paper Details

Date Published: 13 February 1997
PDF: 8 pages
Proc. SPIE 3054, Optoelectronic and Electronic Sensors II, (13 February 1997); doi: 10.1117/12.266713
Show Author Affiliations
Dorota Pijanowska, Institute of Biocybernetics and Biomedical Engineering (Poland)
Wladislaw Torbicz, Institute of Biocybernetics and Biomedical Engineering (Poland)


Published in SPIE Proceedings Vol. 3054:
Optoelectronic and Electronic Sensors II
Zdzislaw Jankiewicz; Henryk Madura, Editor(s)

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