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Proceedings Paper

Pump-during-probe fluorometry of phytoplankton: group-specific photosynthetic characteristics from individual cell analysis
Author(s): Alexander M. Chekalyuk; Robert J. Olson; H.M. Sosik
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Paper Abstract

Saturating-flash fluorescence techniques are used to monitor the state of the photosynthetic apparatus in phytoplankton under natural conditions. At present these are bulk water measurements, which produce estimates of average properties of all the fluorescent particles present in a sample. Here we describe an improved approach for single-cell measurements of phytoplankton. We have combined individual- cell 'pump-during-probe' measurements of chlorophyll fluorescence induction on the time scale of 30 to 100 microseconds with flow cytometric characterization of each cell, to obtain population-specific photosynthetic characteristics. The results provide information about the potential quantum yield of photochemistry, the fraction of functional reaction centers, and the functional absorption cross section for photosystem 2.

Paper Details

Date Published: 6 February 1997
PDF: 6 pages
Proc. SPIE 2963, Ocean Optics XIII, (6 February 1997); doi: 10.1117/12.266411
Show Author Affiliations
Alexander M. Chekalyuk, Woods Hole Oceanographic Institution (United States)
Robert J. Olson, Woods Hole Oceanographic Institution (United States)
H.M. Sosik, Woods Hole Oceanographic Instititution (United States)

Published in SPIE Proceedings Vol. 2963:
Ocean Optics XIII
Steven G. Ackleson; Robert J. Frouin, Editor(s)

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