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Proceedings Paper

Design and characterization of a phantom that simultaneously simulates tissue optical properties between 400 and 650 nm
Author(s): Georges A. Wagnieres; Shangguan Cheng; Matthieu Zellweger; Nora Doegnitz-Utke; Daniel Braichotte; Jean-Pierre Ballini; Hubert van den Bergh
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Paper Abstract

The design and characterization of optical phantoms which have the same absorption and scattering characteristics as biological tissues in a broad spectral window (between 400 and 650 nm) are presented. These low cost phantoms use agarose dissolved in water as the transparent matrix. The latter is loaded with various amounts of silicon dioxide, intralipid, ink, bovine serum, blood, azide, penicillin and fluorochromes. The silicon dioxide and intralipid particles are responsible for the light scattering whereas the ink and blood are the absorbers. The penicillin and the azide are used to insure the conservation of such phantoms when stored at 4 degrees Celsius. The serum and fluorochromes, such as Coumarin 30, produce an autofluorescence similar to human tissues. Various fluorochromes or photosensitizers can be added to these phantoms to simulate a photodetection procedure. The absorption and fluorescence spectroscopy of the dyes tested was not different in these phantoms than in live tissues. The mechanical properties of these gelatinous phantoms are also of interest as they can easily be molded and reshaped with a conventional cutter, so that for instance layered structures, with different optical properties in each layer, can be designed. The optical properties of these phantoms were determined between 400 and 650 nm by measuring their effective attenuation coefficient ((mu) eff) and total reflectance (Rd). The microscopic absorption and reduced scattering coefficients ((mu) a, (mu) s') were deduced from (mu) eff and Rd using a Monte-Carlo simulation.

Paper Details

Date Published: 6 December 1996
PDF: 10 pages
Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260824
Show Author Affiliations
Georges A. Wagnieres, Ecole Polytechnique Federale de Lausanne (Switzerland)
Shangguan Cheng, Ecole Polytechnique Federale de Lausanne (Switzerland)
Matthieu Zellweger, Ecole Polytechnique Federale de Lausanne (Switzerland)
Nora Doegnitz-Utke, Ecole Polytechnique Federale de Lausanne (Switzerland)
Daniel Braichotte, Ecole Polytechnique Federale de Lausanne (Switzerland)
Jean-Pierre Ballini, Ecole Polytechnique Federale de Lausanne (Switzerland)
Hubert van den Bergh, Ecole Polytechnique Federale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 2926:
Optical Biopsies and Microscopic Techniques
Irving J. Bigio; Warren S. Grundfest M.D.; Herbert Schneckenburger; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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