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Proceedings Paper

Novel laser-based technology for cell separation
Author(s): Edgar Diessel; Franco Fois; Ulrich Ebbinghaus-Kintscher; Juergen Stebani
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Paper Abstract

A UV laser-based separation technique for biological cells is presented. This method refers to the separation of cells that adhere to a special UV absorptive polymer foil. To separate a cell the UV microbeam excises a disc with the attached cell out of the foil. Then, the beam transfers this complex to a separate substrate. This principle allows a rapid separation of adherent cells without the risk of contamination. The operating conditions of the separation scheme were examined. The laser separation scheme was applied to the separation of human embryonic kidney (HEK- 293) cells. The viability tests revealed that these cells survived the procedure. Therefore, the laser separation method is considered a useful tool for separating biological cells.

Paper Details

Date Published: 6 December 1996
PDF: 10 pages
Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260810
Show Author Affiliations
Edgar Diessel, Bayer AG (Germany)
Franco Fois, Bayer AG (Germany)
Ulrich Ebbinghaus-Kintscher, Bayer AG (Germany)
Juergen Stebani, Bayer AG (Germany)


Published in SPIE Proceedings Vol. 2926:
Optical Biopsies and Microscopic Techniques
Irving J. Bigio; Warren S. Grundfest; Herbert Schneckenburger; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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