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Proceedings Paper

Imaging of living cells in real time
Author(s): Vladimir P. Tychinsky; Georgy E. Koufal; Elena V. Perevedentseva; Tatiana V. Vyshenskaia; Serguei L. Nikandrov
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Paper Abstract

Parameters of intrinsic cell motility is one of the cell activity characteristics which can be measured in real-time. For evaluation of certain organelles velocity we propose to use high sensitivity of computer-aided phase microscope airyscan to local phase changes connected with refractive index. This method is based on periodical scanning of cell profile in direction perpendicular to organelles movement. Analysis of the obtained 2-dimensional time-coordinate matrix allows us to define organelle velocity in quasi-real time and areas of cell activity. The experiments with onion cells confirm the method applicability for cell activity investigation.

Paper Details

Date Published: 6 December 1996
PDF: 8 pages
Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260807
Show Author Affiliations
Vladimir P. Tychinsky, Moscow Institute for Radioengineering, Electronics and Automation (Russia)
Georgy E. Koufal, Moscow Institute for Radioengineering, Electronics and Automation (Russia)
Elena V. Perevedentseva, Moscow State Institute of Radioengineering, Electronics and Automation (Russia)
Tatiana V. Vyshenskaia, Moscow Institute for Radioengineering, Engineering and Automation (Russia)
Serguei L. Nikandrov, Moscow State Institute of Radioengineering, Electronics and Automation (Russia)


Published in SPIE Proceedings Vol. 2926:
Optical Biopsies and Microscopic Techniques
Irving J. Bigio; Warren S. Grundfest; Herbert Schneckenburger; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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