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Proceedings Paper

Comparative study viruses with computer-aided phase microscope AIRYSCAN
Author(s): Vladimir P. Tychinsky; Georgy E. Koufal; Elena V. Perevedentseva; Tatiana V. Vyshenskaia
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Paper Abstract

Traditionally viruses are studied with scanning electron microscopy (SEM) after complicated procedure of sample preparation without the possibility to study it under natural conditions. We obtained images of viruses (Vaccinia virus, Rotavirus) and rickettsias (Rickettsia provazekii, Coxiella burnetti) in native state with computer-aided phase microscope airyscan -- the interference microscope of Linnik layout with phase modulation of the reference wave with dissector image tube as coordinate-sensitive photodetector and computer processing of phase image. A light source was the He-Ne laser. The main result is coincidence of dimensions and shape of phase images with available information concerning their morphology obtained with SEM and other methods. The fine structure of surface and nuclei is observed. This method may be applied for virus recognition and express identification, investigation of virus structure and the analysis of cell-virus interaction.

Paper Details

Date Published: 6 December 1996
PDF: 4 pages
Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260806
Show Author Affiliations
Vladimir P. Tychinsky, Moscow Institute for Radio Engineering, Electronics, and Automation (Russia)
Georgy E. Koufal, Moscow Institute for Radio Engineering, Electronics, and Automation (Russia)
Elena V. Perevedentseva, Moscow Institute for Radio Engineering, Electronics, and Automation (Russia)
Tatiana V. Vyshenskaia, Moscow Institute for Radio Engineering, Electronics, and Automation (Russia)


Published in SPIE Proceedings Vol. 2926:
Optical Biopsies and Microscopic Techniques
Irving J. Bigio; Warren S. Grundfest; Herbert Schneckenburger; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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