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Proceedings Paper

Optical determination of motility forces in human spermatozoa with laser tweezers
Author(s): Karsten Koenig; Lars Othar Svaasand; Yona Tadir; Bruce J. Tromberg; Michael W. Berns
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Paper Abstract

Laser tweezers may act as optical force transducers. We report on the determination of intrinsic motility forces of human spermatozoa by employing an 800 nm optical trap. The cellular forces were calculated from calibrated trapping forces. The determination of trapping forces based on a hydrodynamic model for ellipsoidal specimens, the measurement of the minimum laser power required to confine a single cell in the trap, and the calculation of viscus forces during the movement of optically trapped sperm heads through a laminar fluid. A mean motility force of 44 plus or minus 24 pN was calculated for spermatozoa of healthy donors.

Paper Details

Date Published: 6 December 1996
PDF: 6 pages
Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260803
Show Author Affiliations
Karsten Koenig, Institute of Molecular Biotechnology (Germany)
Lars Othar Svaasand, Univ. of Trondheim (Norway)
Yona Tadir, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 2926:
Optical Biopsies and Microscopic Techniques
Irving J. Bigio; Warren S. Grundfest; Herbert Schneckenburger; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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