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Proceedings Paper

Autofluorescence properties of normal and transformed fibroblasts
Author(s): Anna Cleta Croce; Alessandra Spano; Piera Balzarini; Donata Locatelli; Sergio Barni; Proto Pippia; Giovanni F. Bottiroli
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Paper Abstract

The autofluorescence properties of single living cells were studied through microspectrofluorometric analysis under different biological conditions. A tumor cell line, derived from a spontaneous sarcoma of a Galliera strain rat (SGS/3A), and normal fibroblasts (FG) at different culture passage numbers, derived from syngeneic rats, were used as a model. Under excitation at 366 nm autofluorescence is mainly due to the presence of coenzymes: NAD(P)H in the 440 - 480 nm spectral region, and flavines and lipopigments at wavelengths longer than 510 nm. Differences in the fluorescence emission amplitude and spectral shape are found in dependence on cell metabolic activity and transformation conditions.

Paper Details

Date Published: 6 December 1996
PDF: 5 pages
Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260801
Show Author Affiliations
Anna Cleta Croce, Univ. di Pavia (Italy)
Alessandra Spano, Univ. Sassari (Italy)
Piera Balzarini, Univ. di Pavia (Italy)
Donata Locatelli, Univ. di Pavia (Italy)
Sergio Barni, Univ. di Pavia (Italy)
Proto Pippia, Univ. Sassari (Italy)
Giovanni F. Bottiroli, Univ. di Pavia (Italy)


Published in SPIE Proceedings Vol. 2926:
Optical Biopsies and Microscopic Techniques
Irving J. Bigio; Warren S. Grundfest; Herbert Schneckenburger; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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