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Proceedings Paper

Cell damage in two-photon microscopes
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Paper Abstract

We report on power thresholds for cell damage in femtosecond two-photon NIR microscopes and discuss interactions between vital cells and high intense femtosecond kW pulses in the TW/cm2 (1012 W/cm2) range. The influence of femtosecond NIR pulses on cell metabolism and cell vitality was studied by autofluorescence imaging, morphology studies, employment of vitality kits and sensitive cell cloning assays. We show that cells remain unaffected by high intense NIR femtosecond pulses below certain power thresholds. This allows nondestructive nonlinear 3D fluorescence imaging of vital cells. Above these thresholds, giant cell growth, failed cell division, complete cell destruction, and intracellular plasma formation occur.

Paper Details

Date Published: 6 December 1996
PDF: 5 pages
Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); doi: 10.1117/12.260794
Show Author Affiliations
Karsten Koenig, Fredrich-Schiller-Univ. (Germany)
Peter T. C. So, Univ. of Illinois/Urbana-Champaign (United States)
William W. Mantulin, Univ. of Illinois/Urbana-Champaign (United States)
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 2926:
Optical Biopsies and Microscopic Techniques
Irving J. Bigio; Warren S. Grundfest; Herbert Schneckenburger; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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