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Proceedings Paper

Quantitative analysis of anthracyclines kinetics in cells varying in drug responsiveness
Author(s): Alberto Colasanti; Annamaria Kisslinger; Raffaele Liuzzi; Michele Mastrocinque; Maria Quarto; Patrizia Riccio; Giuseppe Roberti; Fulvia Villani
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Paper Abstract

A fluorimetric technique to measure transport parameters of fluorescent drugs through cellular membranes is described. This method eliminates procedures that would lead to errors in the measurement of drug accumulated by cells, and measures the fraction of free drug in cells. The use of a simple three-compartment model in conjunction with fluorescence measurements performed on the extracellular medium and on Triton-permeabilized cells at different times during daunorubicin incorporation allows determination of the kinetic parameters of the transport through cellular membranes. With this technique we found that LoVo cells have a greater daunorubicin uptake, a similar input rate constant and a lower output rate constant than the drug resistant LoVo/DX cells. Preliminary photoactivation measurements of these two cell lines with these compounds showed that phototoxic effects are related to the amount of drug bound to cellular sites.

Paper Details

Date Published: 4 December 1996
PDF: 7 pages
Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); doi: 10.1117/12.260777
Show Author Affiliations
Alberto Colasanti, Univ. of Naples and Istituto Nazionale Fisica della Materia (Italy)
Annamaria Kisslinger, Univ. of Naples (Italy)
Raffaele Liuzzi, Facolta di Medicina et Chirurgia/Univ. of Naples and Istituto Nazionale Fisica della Mater (Italy)
Michele Mastrocinque, Univ. of Naples (Italy)
Maria Quarto, Istituto Nazionale Fisica della Materia (Italy)
Patrizia Riccio, Facolta di Medicina et Chirurgia/Univ. of Naples and Istituto Nazionale Fisica della Mater (Italy)
Giuseppe Roberti, Facolta di Medicina et Chirurgia/Univ. of Naples and Istituto Nazionale Fisica della Mater (Italy)
Fulvia Villani, Facolta di Medicina et Chirurgia/Univ. of Naples and Istituto Nazionale Fisica della Mater (Italy)


Published in SPIE Proceedings Vol. 2924:
Photochemotherapy: Photodynamic Therapy and Other Modalities II
Stanley B. Brown; Benjamin Ehrenberg; Johan Moan, Editor(s)

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