Share Email Print

Proceedings Paper

Spectroscopic studies of photosensitizer-human serum albumin complexes and their photostability
Author(s): A. Jasaitis; Giedre Streckyte; Ricardas Rotomskis
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The interaction of haematoporphyrin (Hp), dimethoxyhematoporphyrin IX (DMHp), meso-tetraphenylporphine tetrasulphonate (TPPS4), photofrin II (PF), chlorin e6 (Cle6) and aluminum phthalocyanine tetrasulphonate (AlPcS4) with human serum albumin (HSA) in aqueous solution and the influence of complexation on the photostability of sensitizers have been investigated by means of steady state absorption spectroscopy. By using the binding isotherm method and graphical Scatchard plot the sequence of affinity of sensitizers to HSA was obtained: TPPS4 greater than DMHp, Cle6, Hp, AlPcS4 greater than PF. The influence of aggregation on the binding ability of sensitizers is discussed. It was observed that complexation with HSA decreases the photostability of sensitizers. The increased rates of sensitizer photobleaching in the presence of photo-oxidizable substrates can be explained by the changes of photophysical properties of sensitizers caused by the interaction with substrates or by the attack of the biomolecules photo- oxidized by the type I mechanism involving electron transfer from excited porphyrin molecule or by the type II mechanism via singlet oxygen on porphyrin microcycle. The sequence of photostability of sensitizers in the presence and in the absence of HSA was established.

Paper Details

Date Published: 4 December 1996
PDF: 11 pages
Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); doi: 10.1117/12.260770
Show Author Affiliations
A. Jasaitis, Vytautas Magnus Univ. (Lithuania)
Giedre Streckyte, Laser Research Ctr./Vilnius Univ. (Lithuania)
Ricardas Rotomskis, Laser Research Ctr./Vilnius Univ. (Lithuania)

Published in SPIE Proceedings Vol. 2924:
Photochemotherapy: Photodynamic Therapy and Other Modalities II
Stanley B. Brown; Benjamin Ehrenberg; Johan Moan, Editor(s)

© SPIE. Terms of Use
Back to Top