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Proceedings Paper

Photodynamic treatment with zinc poly-substituted phthalocyanines causes the release of tissue factor in an endothelial cell line
Author(s): Janet E. Cruse-Sawyer; B. Dixon; John Griffiths; Stanley B. Brown
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Paper Abstract

Tissue factor (TF) is not expressed by endothelial cells unless they have ben perturbed. Identifying the extrinsic pathway of coagulation as the process involved in clot formation demonstrated that PDT induces release of TF by endothelial cells. EAhy 926 cells were incubated with 10 (mu) g ml-1 of photosensitizer for 24 h prior to illumination at 40 J cm-2. A clotting assay was then performed. PDT caused a significant reduction in clotting time. This was repeated using factor VII- or X-deficient serum. In factor VII-deficient serum there was no difference between treated and untreated cells. There was no clot formation with any cells in the presence of factor x- deficient serum. The data indicate that PDT-induced procoagulation activity is via the extrinsic pathway of coagulation and results from direct initiation of endothelial cells to release tissue factor.

Paper Details

Date Published: 4 December 1996
PDF: 7 pages
Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); doi: 10.1117/12.260757
Show Author Affiliations
Janet E. Cruse-Sawyer, Research School of Medicine/Univ. of Leeds (United Kingdom)
B. Dixon, Research School of Medicine/Univ. of Leeds (United Kingdom)
John Griffiths, Univ. of Leeds (United Kingdom)
Stanley B. Brown, Univ. of Leeds (United Kingdom)


Published in SPIE Proceedings Vol. 2924:
Photochemotherapy: Photodynamic Therapy and Other Modalities II
Stanley B. Brown; Benjamin Ehrenberg; Johan Moan, Editor(s)

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